Using of Immunohistochemistry Technique for Detection of Foot and Mouth Disease Virus (FMDV) Type O in Cattle Tissues
نویسندگان
چکیده
Detection of FMDV type O antigen using Immunohistochemistry (IHC) technique was performed on formalin-fixed, paraffin-embedded (FFPE) tissues obtained from cattle originally intradermolingual inoculated with FMDV type O strain O Udornthani/87, FMDV type A strain A Lopburi/2012 and FMDV type Asia-1 strain Asia-1 Nakornpathom/85 to consider the cross reaction. The number of animals used for these inoculations were 5, 2 and 2 healthy 6 month old cattle respectively. The primary antibody used in this study was mouse against FMDV type O monoclonal antibody. The positive IHC results were only found in cattle tissues inoculated with FMDV type O strain O Udornthani/87 which moderate to strong positive results were observed in the epithelial cells of sloughing and necrotic tongue epithelium (5/5) and stratum spinosum and stratum basale of the interdigital skin (2/5), in addition, the mild positive results were noticed in a few epithelial cells of the dorsal soft palate (1/5). The important microscopic changes in the organs and tissues of cattle inoculated with FMDV type O strain O Udornthani/87 were necrosis with sloughing and sub-acute to chronic inflammation of tongue (5/5), interdigital skin (3/5), gum (2/5) and larynx (2/5) and chronic inflammation of epiglottis (1/5) and dorsal soft palate (1/5). From the current study, IHC technique showed magnificent results to detect the antigen of FMDV type O without cross reaction, in addition, the IHC positive reaction findings showed the ability of mouse against FMDV type O monoclonal antibody to particularly detect the antigen of the virus even small amounts in FFPE tissues. Furthermore, tongue and interdigital skin with sloughing and necrosis were significant tissues to detect more FMDV antigen. In conclusion, the IHC technique is an alternatively diagnostic method for detection of FMDV antigen since it is specific, sensitive and able to be performed completely within 2 days after obtaining FFPE tissue blocks.
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